One of the proteins in S.pombe that I am working on is tagged with FLAG, so can I still call this strain as wild type as it contains a fragment of foreign DNA? If no, what is the right term for it?
Sven Reischauer
No, you created an allele. That said, you should give it an allele number (according the yeast nomencleature) and thats how you will call it.
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Martin Klvana
The FLAG-tag is attached to the wild-type sequence; the FLAG-tag does not modify the wild-type sequence. Hence: "FLAG-tagged wild-type protein".
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Dominique Liger
Hi there,
it's not wt anymore as it is genetically modified though its phenotype might still be wt.
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