I have 8 sequences of the enzyme creatine kinase from organism A. I am interested in obtaining a consensus sequence to later use it as a template to search for this sequence in the genome of organism B.
https://www.bioinformatics.nl/cgi-bin/emboss/cons
I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...
28 February 2021 606 3 View
I'm working on a study of how selection criteria of Tourism have changed considering Pandemic + Vaccination expectation, so propose a new prediction behavioral dynamic model for Riviera Maya (Mexico).
09 February 2021 314 7 View
Hello everybody! I have performed an acidic MeOH:HCl 1% (v/v) plant extraction from Arabidopsis leaves to calculate Anthocyanins concentrations based on: Rabino, I., and Mancinelli, A. L. 1986....
25 January 2021 3,686 2 View
Cronbach's alpha or McDonald's omega? When it comes to reliability, we find different methods for its estimation. Immediately, the name of Cronbach's Alpha coefficient arises, as it is the most...
21 January 2021 330 4 View
We have a projet about TAVI
22 December 2020 7,729 1 View
I noticed that in many papers each author has a different human dummy to perform CFD simulation. However shouldn't this be standardize according to anthropomorphic data available? Right now, I...
12 December 2020 8,746 3 View
I been wondering if genes when stable transfected become regulated by the cell, or if the gene is constitutively express and translated and the protein previously made is degraded at a slower rate...
30 November 2020 8,095 2 View
I have used ICA for the problem of mixing acoustic and UHF signals from partial discharges. I know that it has been used in Biomedical, Acoustic, and many other fields. Do you comment on how you...
09 November 2020 630 2 View
To study the toxicity of a contaminated soil with petroleum and heavy metals using human cell lines as model organism, we need to perform a pollutant extraction step before carrying out the...
08 November 2020 1,421 4 View
I would like to ask a recommendation of a statistical test to answer a question related to an unusual data structure. I am testing if birds change nest parental care behavior depending on...
17 October 2020 2,026 1 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hello, I need your help. I am looking for a database of antibody amino acid sequences for later simulation in a program in order to analyze their structure. The antibodies I need to test...
18 February 2021 371 4 View
I am doing multiple sequence alignment to design primer for my gene. Can anyone tell me how to select the consensus sequence after alignment. I am using ClustalX for the alignment.
04 February 2021 8,872 2 View
I am trying to find a suitable buffer for storage of a His-tag purified protein. The amino acid sequence is pasted below. Can anyone help please? MMSFVSLLLV SILFPAIQAK QFTKCELPQV LKDMDGFGGI...
01 February 2021 5,216 4 View
I want to compare the RBD of all pathogenic viruses? Is there a database or manuscript that anyone knows of where I can access these?
25 January 2021 3,843 4 View
To perform the Luciferase assay for microRNA-gene interaction its necessary a primer (wild-type) to amplificate the specific ligation site and also a mutated primer. But if your primer has a...
19 January 2021 8,568 3 View
We use an AB 3130xl Genetic Analyzer for Sanger Sequencing, and on occasion, we see our plasmid samples struggle with wavy, loss of resolution (as seen in the attached image). Our general...
14 January 2021 7,346 6 View
Each time this SSR primer show band like that other show one or two band . Is it polymorphic ?
11 January 2021 2,221 5 View
I have an inducible expression of the enzyme in a newly derived bacterial strain, and methylation may be affected. I want to test methylation efficiency with a bisulfite kit. The kit is the EZ DNA...
06 January 2021 7,218 2 View
I want to predict the protein 3D structure by using computational tools. In this case, amino acid sequences are available only. What software would be best with maximum accuracy to predict this...
01 January 2021 444 3 View