If i fix my root sample over night in 4% glutaraldehyde, does this have an affect on the morphology or anatomy of sample?

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Prakash S Bisen

It should work well.

Yasmin Bano

yes you can use. i also did it.

Sanketh Dhumal Satya

The extensive cross-linking adversely affects immunohistochemical staining but does provide excellent ultrastructural preservation which explains its extensive use as a primary fixative for electron microscopy. Cross-linking reactions of glutaraldehyde are largely irreversible. Glutaraldehyde penetrates very slowly and it is recommended that tissue be less than 1mm in thickness in at least one dimension