Used but not for metabolite. Whats the problem that you are facing ?
Article The use of spin desalting columns in DMSO-quenched H/D-excha...
If you're concerned about calculating acidity, you could go the protein route and use solvent suppression techniques. Many protein NMR samples use a 90:10 ratio of H2O:D2O. This would allow you to better approximate pH if using NaOH and HCl with H2O before diluting with D2O.
More info here for comparison of techniques.
http://nmr.chem.indiana.edu/nmrblog/?page_id=989
If you want to adjust the pH, using NaOD and DCl (not HCL) use the relationship between pH and pD first: pD=pH+0.39. Also use constant ionic strength and temperature.
Thank you all. I need to follow below referred protocol for adjustment of pH.
see the link: Article Metabolite profiling of the intraerythrocytic malaria parasi...
The freeze-dried extracts were dissolved in 600 mL D2O with
sodium trimethylsilyl-2,2,3,3-tetradeuteropropionate (TSP) added
at a known concentration (in the range 0.1–0.2 mM). TSP served
as both an internal chemical shift reference and as an internal
standard for metabolite quantification. 1H NMR spectra were run
on samples each derived from an extract of (1–4)108 isolated
parasites and adjusted to neutral pH using DCl or NaOD. All
samples were clarified by centrifugation (20 800 g, 5 min) and
550mL aliquots of the supernatant transferred to 5mm NMR tubes
(528-PP Royal Imperial; Wilmad, Buena, NJ, USA) for analysis
There are different NAOD and DCl available online. I was just wondering for which i shd go. DEUTERIUM CHLORIDE (D, 99.5%) DCL 20% W/W SOLUTION IN D2O, DEUTERIUM CHLORIDE (D, 99.5%) DCL 35% W/W SOLUTION IN D2O, SODIUM DEUTEROXIDE (D, 99.5%) 30% IN D2O, SODIUM DEUTEROXIDE (D, 99.5%) 40% IN D2O.
It dosent matter which one you use all of them are 99.5%, if you use concentrated you need less if you use less concentrated you need more. As you are making 600 ml you can use DEUTERIUM CHLORIDE (D, 99.5%) DCL 35% W/W SOLUTION IN D2O, and SODIUM DEUTEROXIDE (D, 99.5%) 40% IN D2O.
Thanks mahesh, by the way the solution for which i am gone measure the ph (pD) is only 600 microliter. In that i need to add NAOD OR DCl. Off course if i use concentrated one then i need to use very little amount to adjust the pH.
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