Hi everyone, was wondering if I could run something by you all?
I need to identify promoter sequences in several genes of interest as we are interested in doing bisulfite sequencing to determine if CpG islands in the promoter regions are potentially methylated.
I have used ensemble ( ensemble.org) to find the gene sequence before the first exon, and have entered the sequence into UCSC BLAT to determine if the sequence matches with what is on the UCSC database. However I am very new to this kind of work and I am not sure exactly how to interpret results from BLAT. Would anyone be able to help me in identifying gene promoter sequences either through UCSC BLAT or any other recommended software?
I must also say that we are investigating genes in pigs, so most databases/software's that are used for humans and mice cannot be used by us.
Any help at all will be greatly appreciated, many thanks in advance.