Adam Clark

9 Questions 9 Answers 0 Followers

Questions related from Adam Clark

Robust standard errors when performing 2-way ANCOVA?

Hello everyone, I've come across a complication while doing some data analysis and was looking for some advice and interpretations. I need to a run a two way ANCOVA on some data; I'm testing the...

08 February 2021 10,001 1 View

Papers that accept discovery studies?

Hello colleagues, I was wondering if I could ask for general advice or guidance on something? I tried to submit a paper recently, but it got rejected on this occasion. Essentially, we did a...

27 November 2019 4,030 2 View

Sampling femurs for Histology?

Hello everyone, I was just wondering if someone could offer some advice or point me in the direction of a paper that could answer my question? We currently have some femur samples that have been...

19 February 2019 2,448 3 View

General statistics on raw or average values?

Dear All, I was just wondering if I could ask the general community what their approach is in regards to statistical analysis of data? Would you perform statistics on raw data, including...

14 August 2017 2,033 7 View

Identifying gene promoter sequences for methylation study?

Hi everyone, was wondering if I could run something by you all? I need to identify promoter sequences in several genes of interest as we are interested in doing bisulfite sequencing to determine...

14 February 2017 5,863 3 View

Best calibrators for qPCR when analysing disease?

Hi everyone, I was wondering if I could please receive some feedback with something? I am currently investigating what genes are being up regulated/down regulated in pigs suffering from a...

06 May 2016 5,103 10 View

Will increasing cDNA reaction volume allow cDNA synthesis from low volumes of RNA?

Hi everyone, I was wondering if I could please run something by you all and gain some feedback? I am required to synthesise cDNA from RNA for qPCR experiments, so far we've been able to get a...

25 February 2016 4,397 3 View

Why doI get late Ct values in qPCR linked to cDNA synthesis?

Hello All. I am not entirely sure if what I am seeing is a problem but I wanted to get it out there and see if there this is a problem which I will need to resolve. I am doing a lot of qPCR's at...

07 October 2015 9,777 8 View

Is it possible to design primers from predicted sequences?

I need to design primers to amplify and quantify cDNA targets in a qPCR assay, the cDNA of which has been reverse transcribed from isolated RNA from pig tissues. However the mRNA sequences for...

09 June 2015 2,918 3 View