How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Will it be possible to get IC:50 if we target cancer cell line with antibody drug conjugate (ADC) that have 50% less antigen positive cells ?

Is it possible to attain IC50 if my target cells have less than 50% antigen expression ? For example: Cancer cell line XXX is showing 35% of expression of cell surface antigen YYYY, if i...

04 February 2021 2,711 3 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

IC50 value from this graph?

01 January 2021 6,848 4 View

I am performing enzyme inhibition assay and the substrate is fluorogenic & fluorescence was measured. Now I want to calculate Vo and Vi. How can I ??

These are the per minute fluorescence observed. Please guide me how can I determine Vi, Vo and IC50 ? By slope determination or any other method? Control: 28.11 40.30 40.78 37.61...

11 December 2020 1,037 4 View

How to determine non-cytotoxic drug concentration using MTT assay?

Many literature showed that how to determine IC50 drug concentration using MTT assay. But I want to know how to determine non-cytotoxic concentration of a particular drug with maximum its...

31 October 2020 2,012 3 View

How do I find the IC50 and best drug treatment time for anticancer drug?

I want to find the IC50 of a drug on a cancer cell line. I'm not sure how long the treatment time should be for in vitro experiment. Is there an assay/protocol I can follow to determine the IC50...

15 October 2020 8,678 6 View

Are you using pIC50 instead of IC50?

Hi, An IC50 is the concentration at which a ligand (drug, extract, ...) can inhibit an enzyme by 50%. pIC50 is a new approach to examine the same data in a logarithmic manner. I ask the RG...

24 September 2020 1,531 3 View

IC50 for plant extracts?

If I am working with plant extracts and their photoprotective properties.... It is just enough to evaluate the citotoxicity at a specific (high) concentration of my extracts or it is necessary to...

14 September 2020 8,890 1 View

What is the correct standard IC50 limit for a crude plant extract in cancer cell ?

i want a stander ic50 for plant to know about the strong of my crud extract plant in cancer cell line

24 July 2020 2,461 2 View