I would like to localize an enzyme in cytoplasm and nucleus. Does anyone have a protocol to share with me?

16 September 2022 0 5K Report

I am doing research in cell biology and studying a specific pathway. I am working with cell lines and am studying an enzyme and need to localize it in the cytoplasm and nucleus.

Badges
Science topic

More Elar Ad's questions See All

Can we say that the traditional Arabic linguistic theory is similar to the Western linguistic theory?

There are many differences between them in general intellectual, epistemological and cultural characteristics. Among the important sources on the subject is the book of Dr. Abd al-Salam al-Masdi...

15 December 2022 9,957 2 View

PhD Behavioural Finance Research Propsal?

Hi All, I am considering a PhD in Finance and would like suggestions on a research proposal ( Behavioural finance and investing).

18 August 2022 5,551 6 View

Can I use Hot start Q5 polymerase enzyme in Digital PCR?

I am using Q5 hot start DNA polymerase in RT-PCR and am able to generate good results, however, whenever i apply it in ddPCR i am not getting any results. I am aware of optimization, however, I...

29 December 2021 8,106 4 View

Hi, does toxic tetraethyl ammonium acetate (TEAA) salt have any impact on RT-qPCR probes?

I ordered probe and it has HPLC purification and phosphorothioate bonds. should I add Na+ exchange processing to ensure that residual toxic TEAA (Tetraethyl ammonium acetate) salts from HPLC...

16 September 2021 5,065 0 View

ما الذي يسببه الغضب من اثار صحية وسلوكية على الانسان ؟ | What does anger cause health and behavioral effects on humans?

It is known that anger is a psychological reaction that affects a person because of a reaction to something, and therefore, if anger attacks occur, what can they cause in terms of psychological...

05 October 2020 4,596 3 View

Which surfactant is most effective in fighting the COVID-19 virus?

It should matter which type of surfactant works best. Obviously some cationic types will do the job, but may have toxicity concerns. IS there a difference between sulphonates/sulphates and...

22 April 2020 1,908 3 View

In the light of what we see as a multiplicity in the phonetic images in reading Qur'an (Qur'anic Readings). Is there a difference between Holy Qur'an?

In the light of what we see as a multiplicity in the phonetic images in reading Qur'an (Qur'anic Readings). Is there a difference between Holy Qur'an, revealed by Allah to His Messenger Muhammad...

02 February 2019 9,897 7 View

Why they aren't any migration of the B cation (Pb/Sn) in the perovskite solar cells ?

up to now i found that the most of the studies about the migration in the perovskite solar cells they talk about the migration of the A cation and the X anion , for the B cation they are a...

22 January 2019 8,940 1 View

Is it valid to model one explanatory variable with many instrumental variables?

If we find that one explanatory variable is influenced by possible omitted factors, can we model that with many instrumental variables? In case that it is valid to do that, can anyone provide some...

18 January 2019 5,450 1 View

Is it valid to model one explanatory variable with many instrumental variables?

If we find that one explanatory variable is influenced by possible omitted factors, can we model that with many instrumental variables? In case that it is valid to do that, can anyone provide some...

31 December 2018 4,145 1 View

Why don't d-orbitals split themselves, why does it take a ligand? why don't protons from ligand repel nucleus split d-orbitals?

why don't d-orbitals split themselves because of themselves without the presence of ligands? Electrons are indistinguishable. Why wouldn't it be more correct that protons from a ligand split the...

03 August 2024 3,589 3 View

Why doesn't chromium 2+ ion use all its d-orbitals to receive lone pairs from six waters in [Cr(H20)6)]3+?

I'm guessing it's because the ligand experiences too much electron repulsion or proton repulsion from the chromium to insert them close to the 3d-orbitals which are close to the metal nucleus. Is...

03 August 2024 1,370 1 View

While using the IHC PROFILER plugin in image j software, to quantify the epithelial cytoplasmic DAB staining do you crop remove the stromal tissue?

My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...

29 July 2024 2,682 6 View

How to perform immunofluorescence on suspension cells?

Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...

20 July 2024 3,797 2 View

Why I have this weird green signal in the nucleus?

Hi, In a few wells and cells, we’ve noticed an unusual signal in the nucleus using both eppifluroscence & confocal microscopes. Does anyone know why this is happening? It occurred twice in...

10 July 2024 1,870 0 View

What does it mean when NucBlue disappears completely during live cell imaging?

Using confocal imaging, I'm investigating the effect of a particular compound on mitochondrial membrane potential in live cells. I've stained the cells with varying concentrations of the compound...

29 June 2024 7,546 4 View

Does anyone have experience with immunofluorescence of endoplasmatic reticulum?

Hi! I have performed several immunofluorescences of cultured ARPE-19 cells with different antibodies expecting in each one a cytoplasmic localization. However, it seems to localize in the...

06 June 2024 7,764 1 View

Is CFSE retained in cell nuclei?

Does anybody know whether CFSE is retained in cell nuclei after treatment of cells with pepsin solution in HCl?

29 May 2024 1,357 0 View

Is this mycoplasma contamination?

I had mammalian cancer cell line cultured and fixed for confocal imaging. While observing I found these particles around cell nuclei stained with dapi. I checked the main culture for bacteria but...

15 May 2024 9,102 2 View

Are they nuclear debris?

This is from an endomyocardial biopsy from a 24 year old male with clinical hypertrophic cardiomyopathy. However, no mutation is found in the related genes. What are the irregular nuclear-like...

12 May 2024 8,148 1 View