How to assess scale reliability and validity?

I am conducting a qualitative study which i need to develop a scale, I need to know how to assess the reliability and validity of this scale?

08 September 2017 3,602 6 View

Are there any differences in calculation of Credit hours in undergraduates and postgraduates?

09 October 2016 290 0 View

Is it necessary to teach bioinformatics for undergraduate medical students?

06 July 2016 3,590 3 View

Regarding statistical data, why Epidata as software is preferable than SPSS?

what are the pros of Epidata compare to SPSS in handling the data set.

11 December 2015 3,148 0 View

Are scientist biased to their race or nations?

Are scientist thinking could be based on their race when write a scientific article, cite their race result and papers rather than others result, hide some result that may insult their race and...

10 November 2015 9,749 5 View

Is there any software that can predict the effect of the SNPs in the 3D of the protein?

09 October 2015 8,840 8 View

How can i know the genetic biological network interactions for a particular disease?

08 September 2015 3,245 0 View

Are there any way to determine the exact cause of genotyping deviation from Hardy Weinberg equilibrium (HWE)?

It is well known that, there are many causes of deviation of the genotyping from the HWE. However, i wounder are there any way to know excatly the cause of this deviation from HWE.

08 September 2015 6,029 14 View

Any one can help in how to make standerdized psychometric test ?

08 September 2015 2,172 5 View

How can i quantifi an enzyme which expressed in tissues not in blood cells?

I try to measure the enzyme concentration for the NO3 which is an endothelial enzyme expressed mainly in the endothelial tissues, some literature report measuring from blood samples, but is it a...

08 September 2015 5,031 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

How to visualise pathways from a phosphoproteomics dataset?

I am looking for something similar than Ingenuity Pathway Analysis (IPA) in which I can enter the proteins differentially phosphorylated and I can visualise the pathways these proteins are...

21 February 2021 8,923 4 View

Which CpGs have a significant effect on gene expression?

It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...

20 February 2021 5,634 3 View