I am going to infect suspension cell line (SKW, Normal B-Lymphocytes) with mutant K-RAS, I would like to ask for advises to increase the infection efficiency of the suspension cell.
If understand correctly, you plan to infect a B cell line with virus (lentivirus?). The critical step is to optimize the ration between B cells and virus (1:5 to 1:20). If a selection is involved, select for 1-3 days.