I have strains CCMP1524 and CCMP1528 growing in L1-Si and K-Si media. Cultures are in ambient light and temperature, 22C and 1500Lux, and 18C and 12000Lux, with and without shaking. The cultures are in good condition and reach exponential growth quickly, but will not form colonies!
Albert Calbet
What we did is transfer our culture to filtered sea water with a bit of nutrients (F10, F20 final nutrient concentration) in a 10 L cylinder with an helicoidal paddle that rotated at 10 rpm. What you actually need is to keep the algae in suspension, but in a gentle way. Not by shaking.
I hope this helps
Albert
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