What should I do for my controls not to stain my secondary antibody ( IHC for mice muscle) ?

I am staining mice muscle for macrophages (CD68) and lymphocytes (CD3). The challenge that am facing is that my control is also staining with the secondary antibody even though I did not add the...

15 October 2020 8,139 3 View

Triangulation of data sources in qualitative research: Do you consider it mixed methods research and why, whether its a yes or no?

It is common in qualitative research to interview for example patients and their partners or their health professionals to talk about the same topic and explore the perspectives of all the people...

30 July 2020 538 21 View

How do i determine the sample size for a study looking at the treatment outcomes of mental health patients in a community house ?

How do i determine the sample size for a study looking at the treatment outcomes of mental health patients in a community house that is a step down from inpatient unit. The study will look at...

12 May 2020 7,072 3 View

Who can link me to Cost / Benefit Analysis of Treatment Options for Produced Water of varying quality and quantity?

I work in the oil industry and I wish to focus on reducing reinjection of "free" (non-water right controlled) produced water for beneficial use where economics dictate feasibility. Who can direct...

24 February 2020 3,059 2 View

How is confirmatory factor analysis not just a tautology?

Hello, very new to confirmatory factor analysis (CFA) and structural equation modeling, and I'm trying to understand how to think about confirmatory factor analysis. Suppose I have a construct,...

11 November 2019 3,330 4 View

How to deal with member check conflicts in qualitative research?

Member checking or participant checking is done to validate and improve the credibility of quallitative research results. I am planning to ask my participants to help with that (will ask them to...

27 May 2019 7,799 13 View

How do you create a calibration curve for H2O2 ?

Specifically I'll be looking at H2O2-titanium complexes (in plant tissue) and will be measured @ 415nm. I'm wondering how I can create a calibration curve for the complex... I have 30% H2O2 and...

13 December 2018 4,432 3 View

Improvised abacus? Are there Latin references to betting at Roman amphitheatres, or modern references to Roman use of abaci? Or was it a gaming board?

11 June 2015 1,010 68 View

Do we have instrument development experts among rehabilitation professionals in Sub-Saharan Africa?

It has been noted that clinicians in Africa are forced to use instrument measures that were developed and validated either for American and European children which are quite limited. It is...

01 January 1970 528 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View