What are the methods available to measure the drug-protein interaction?

Hello everyone, I would like to understand the interaction between my drug of interest to a protein (known). Basically, I want to study how strong the interaction is. Is there any way to measure...

10 November 2019 4,636 5 View

How to develop MCF7 mouse xenografts?

Hello everyone, I have two questions, please shed some light on this. I am new to in vivo studies and some may find my questions are trivial. TIA. My first question is, I wanted to develop the...

09 October 2019 4,774 4 View

Can someone please guide me or share the knowledge on how to generate sgRNA pool?

Hello everyone, I have an experience on CRISPR/Cas9-mediated genome editing. So far, I have designed more than 200 guides targeting different human genes and their efficiency have been validated...

06 July 2018 4,309 2 View

Could you please help me why my Flag-tagged gene is not expressing?

Hi all, Whenever I try to do immunoblot for FLAG or HA tag, I could not able to see their expression. Do I need to modify my protocol? I am successfully getting images for endogenous expression....

06 July 2018 9,846 10 View

How to get the information about the list of drugs under the clinical trial for a given disease?

Could you please help me to find the information about the list of drugs under the clinical trial for a given disease? I know my question is vague but any closest answer would be helpful for me....

04 May 2018 6,605 5 View

How to determine the intracellular drug concentration?

Hello all, I wanted to know the amount of drug present inside the cell after drug treatment. I checked articles for the same, finally I ended with targeted mass spectroscopy (MRM analysis). But in...

31 December 2017 2,455 3 View

No band for phosphorylated H2AX (gammaH2AX) when I treat with the drug?

Hi all, I have treated the drug that induces DSB to the cells (HEK293T). After 24 h of incubation, I harvested and did Western blot for gH2AX but I did not see any bands. I made 12% gel and...

11 December 2017 1,087 2 View

Is it necessary to synthesis second strand cDNA to amplify the gene of interest?

Dear All, I have synthesised cDNA first strand using SuperScript III (Invitrogen) and would like to amplify my gene of interest for overexpression studies? Is it necessary to synthesis second...

10 November 2017 9,318 11 View

Can someone please recommend me the protocol for the gamma H2AX foci formation assay?

09 October 2017 8,306 8 View

Problems in amplifying my gene of interest from the cDNA ?

Hello Everyone, I am trying to amplifying a gene from the cDNA. I used Superscript III to prepare cDNA and got 1.5 ug/uL concentration. For amplification, I used 50 ng of cDNA template. But I...

09 October 2017 3,712 6 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View