I want to visualise a protein in the cells by inserting a GFP tag next to the gene so that I get a fusion protein. Any suggestions?
Require for Rietveld refinement in XRD.
08 August 2024 3,081 2 View
I need to do some experiment with it in liquid form.
07 August 2024 613 3 View
AI tools like ChatGPT can enhance research work significantly when used responsibly and in conjunction with thorough human oversight.
05 August 2024 1,842 3 View
How to apply magnetic field parallel to b axis of any crystal
29 July 2024 4,083 2 View
I am looking for journals that admit the publication of results from applying the IUCN Standard for designing and monitoring Nature-based solutions. Many thanks for considering my request.
23 July 2024 6,444 2 View
Large difference in Grain size between the average Grain size(EqCA) Equivalent Circle Area method and average Grain size(Surf) is seen after analyzing the grain size map from ATEX software .
01 July 2024 5,448 3 View
When I examined the GIXRD data obtained recently, I observed that the sample treated with O2 plasma on the Cu surface deposited on Si chips showed increased intensity compared to the sample...
19 June 2024 6,900 5 View
I am working on an acetyltransferase that is highly unstable. Its pI is 6.65, and its molecular weight is around 18 kDa. The protein elutes at 1M imidazole and begins to precipitate immediately...
19 June 2024 2,590 0 View
I conducted GIXRD measurements after performing O2 plasma treatment on a Cu surface. It was observed that the baseline is significantly high at low angles (10-30 degrees). What could this...
18 June 2024 2,996 3 View
I attempted to model a temporary 'lane closure' due to an accident or work zone. Initially, I successfully closed Lane 1 on Link 1, and it worked as expected. However, when I tried to close Lane 1...
17 June 2024 2,070 0 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
average particle size calculation from TEM
04 August 2024 2,921 1 View
I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...
03 August 2024 6,028 4 View