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Questions related from Shivam Mishra
So I am doing a HTS screening to select top drugs for investigating the nucleo-cytoplasmic shuttling of my target protein. The HTS assay involved drug treatment of the cells expressing GFP-tagged...
10 January 2016 195 7 View
My experiment deals with adding a SV40-NLS to my protein of interest but it has already 3 Nuclear Exclusion Signals. I added one NLS signal to the N-terminal of my gene (with a comet-GFP tag),...
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I see a high expression of X protein in spermatogonia and primary spermatocytes and want to check its role in spermatogenesis. For this I propose to isolate spermatoginia from normal mouse testis,...
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Previously I have ordered knockout ES cells for my protein from EUCOMM and we reached the chimeras stage (male and female ~70% approximately). However, I am not getting heterogeneous mice from the...
08 May 2013 250 9 View
Are there any suspension cell lines for breast cancer? For example for cervical cancer we have HelaS3.
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In order to increase the efficiency of knockdown of my protein in cells, I am thinking of using TALEN (transcription activator-like effector nucleases) over shRNA. With the former I get around 75%...
07 February 2013 7,385 0 View
I have made repeated attempts to clone shRNA into pLKO vectors, but have not produced any colonies. I have tried annealing the shRNA in a thermocycler, in PCR, and in a water bath. For vector...
15 January 2013 3,504 16 View
I did a microarray with my protein knockdown in MCF7 cells and found out that the knockdown was just 75% as seen in the uarray. The fold change was also very small - there were just 35 genes with...
01 October 2012 6,901 29 View
