I am currently trying to characterize a small peptide that was designed computationally and later synthesized and purchased from a manufacturer. I want to perform Gas Chromatography-Mass Spectrometry in order to confirm its sequence first but unable to find any proper protocol of recent time has delayed my work. Can anyone help me with a protocol? Could be your own, used for a work and published. Thank you in advance.
The GC-MS sequencing of peptides was pioneered by Klaus Biemann's group and required the derivatisation to N-trifluoroethyl-O-trimethylsilyl polyamino alcohols.
See:
K Biemann. Methods for protein sequencing. ANALYTICAL CHEMISTRY, VOL. 58, NO. 13, NOVEMBER 1986(4) 1288A
Carr, S. A.; Herlihy, W. C.; Biemann, K. Biomed. Mass Spectrom. 1981, 8, 51- 61.
Thank you for the response @ZeyuSun . LCMS is definitely the best choice but given the institution I am in has GCMS but no LCMS, I am planning to anyhow carry out a GC analysis and try my best.
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