I'm currently working with gelatin sponges which have a tendency to float, however, ideally I would like them to remain at the bottom of the well plate as intend to vulture cells on them. Is there an adhesive that I can use in this instance? I would also like to be able to take the sponges out relatively intact for analysis so the adhesive shouldn't be too strong I think.
You might just prepare a batch of gelatin, heat it, dip the bottom of your gelatin sponges, and transfer them to the wells. They should adhere after some time. However, be sure to UV-sterilize everything especially the plates with the sponges attached—before introducing cells and medium.
@Jakub Jagielski
Thank you for your suggestion, I will give that method a try. Although, I have concerns that the gelatin will dissolve over the course of culturing.
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