In Egypt, there is a lack of pure strains of experimental mice. How can I produce a pure strain?
What are the tests that confirm obtaining a pure strain? After how many generations to get a pure strain?
I could get nanoparticles by using a physical method, but because of that method we get different sized molecules and we may not reach the nanometer size on time. How can I determine the size of...
01 February 2019 7,430 20 View
I could get nanoparticles by using a physical method, but because of that method we get different sized molecules. How can I separate the different sizes of nanoparticles?
01 February 2019 8,637 3 View
There are many methods we can make to ensure that the compound becomes in the size of the nanoparticles, most based on devices such as transmission electron microscopy (TEM). Which are not...
31 December 2018 8,825 4 View
The eyes can estimate the concentration of a solution roughly. Through three receptors of colors. Can we estimate the concentration of a substance by measuring its absorption at 3 wavelengths,...
31 December 2018 3,627 3 View
Is there any research papers about increasing the sensitivity of spectrophotometric methods by increasing the length of the optical path?
31 December 2018 4,342 1 View
White mice are characterized by rapid reproduction and rapid growth. Can their meat be used in food industry experiments?
04 May 2018 7,599 5 View
I want to study the effect of temperature on the efficiency of solar cells. So, I needed solar cells in the form of a hollow cylinder to run a stream of water in it. How can I get it and from where?
04 May 2018 6,275 7 View
I want to evaluate some fungicides on some fungus as well as some fungus and bacteria used to biological control of this fungus. How can I evaluate the selectivity of these pesticides?
02 March 2018 1,716 4 View
Since my native language is Arabic, I find difficult to review the linguistic and grammatical errors of my English language. So I want a free program to correct the language and grammar for the...
02 March 2018 4,280 0 View
In Siwa Oasis there are many wells. We want to know the source of water that feeds these wells Is this possible through isotopes for example
31 December 2017 5,000 17 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View