How can I identify the size of nanoparticles without the use of electron microscopy?

01 February 2019 7,263 20 View

How can I separate the different sizes of nanoparticles?

I could get nanoparticles by using a physical method, but because of that method we get different sized molecules. How can I separate the different sizes of nanoparticles?

01 February 2019 8,452 3 View

Is there a simple test we can perform to ensure that we get nanoparticles?

There are many methods we can make to ensure that the compound becomes in the size of the nanoparticles, most based on devices such as transmission electron microscopy (TEM). Which are not...

31 December 2018 8,683 4 View

Can we estimate the concentration of a substance by measuring its absorption at 3 wavelengths, such as what the eyes do?

The eyes can estimate the concentration of a solution roughly. Through three receptors of colors. Can we estimate the concentration of a substance by measuring its absorption at 3 wavelengths,...

31 December 2018 3,471 3 View

The sensitivity of spectrophotometric methods depends on the length of the optical path. How can we increase the optical path length in the sample?

Is there any research papers about increasing the sensitivity of spectrophotometric methods by increasing the length of the optical path?

31 December 2018 4,128 1 View

Can we feed on white rat meat?

White mice are characterized by rapid reproduction and rapid growth. Can their meat be used in food industry experiments?

04 May 2018 7,421 5 View

How can I get solar cells in the form of a hollow cylinder?

I want to study the effect of temperature on the efficiency of solar cells. So, I needed solar cells in the form of a hollow cylinder to run a stream of water in it. How can I get it and from where?

04 May 2018 6,105 7 View

How can I evaluate the selectivity of some pesticides?

I want to evaluate some fungicides on some fungus as well as some fungus and bacteria used to biological control of this fungus. How can I evaluate the selectivity of these pesticides?

02 March 2018 1,554 4 View

What is the best free program to correct grammatical and linguistic for the English language?

Since my native language is Arabic, I find difficult to review the linguistic and grammatical errors of my English language. So I want a free program to correct the language and grammar for the...

02 March 2018 4,138 0 View

How can I know the source of water in a well?

In Siwa Oasis there are many wells. We want to know the source of water that feeds these wells Is this possible through isotopes for example

31 December 2017 4,794 17 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View