I could get nanoparticles by using a physical method, but because of that method we get different sized molecules and we may not reach the nanometer size on time. How can I determine the size of nanoparticles without using an electronic microscope?
Of course there are "no-visualization" techniques that allow subnanometric resolutions in nanoparticles, however this type of statistical and quantitative analysis allows you to know the statistical distribution of the average size of solids, nanoparticles, proteins or whatever, without knowing exactly how they are, the real appearance, their elongation, circularity, sphericity, surface roughness, and many other properties related to their appearance and therefore to the sample visualization.
In dynamic light scattering depending on the equipment and configuration subnanometric resolutions can be obtained, and therefore it is valid to know the distribution of sizes of the sample, but this valid results does not allow you to determine how the sample really is, that is a difference .
Dear Mr Abdallah:
The electron microscopes allow to obtain images with incredible resolutions, perhaps the best that any experimental visualization technique can offer. However, there are other visualization techniques that do not involve the use of the electron microscope and that offer very very good resolutions, such as the Atomic Force Microscopy (AFM) being a technique for the anaysis at the nanoscale.
The AFM in normal conditions imposes few requirements of preparation, under conditions of environmental pressure.
With the AFM it is possible to easily measure objects or structures of nm units, but depending on the microscope, tip, scanner and tip-sample interactions under certain conditions it is possible to obtain sub-nanometer resolution.
In any case, for any microscopic technique , whether it is electron microscopy or Atomic Force Microscopy, obtaining subnanometric resolution is not trivial, to go so far in resolutio, the preparation, cleaning, adjustment of the instrument are crucial to reach the "limit" of resolution, any measuring instrument.
In my opinion the AFM could be an option, at least to explore in a first approximation the characteristics of the sample, if you want to avoid the use of the electron microscope.
Good Luck
C Serra
Dynamic light scattering would be the first option, it will tell you the averaged size and polydispersity of your sample. If you are not sure about your sample size at all, the combined use of small angle x-ray and light scattering could be used to probe sizes over a wide range of length scales. Good Luck.
You can use zetaszier nano system. It’s kind of light scattering teachnique. I have used it many times before.
Regards ...
Of course there are "no-visualization" techniques that allow subnanometric resolutions in nanoparticles, however this type of statistical and quantitative analysis allows you to know the statistical distribution of the average size of solids, nanoparticles, proteins or whatever, without knowing exactly how they are, the real appearance, their elongation, circularity, sphericity, surface roughness, and many other properties related to their appearance and therefore to the sample visualization.
In dynamic light scattering depending on the equipment and configuration subnanometric resolutions can be obtained, and therefore it is valid to know the distribution of sizes of the sample, but this valid results does not allow you to determine how the sample really is, that is a difference .
I have great respect for novice researchers, but when they get more recommendations for a simple question than experts, this is not ethical for researchers.
Yuri Mirgorod
Dr Prof. Mirgorod
You are probably right in what you expose.
I sincerely believe that the number of recommendations are based more on the speed, effectiveness, and "intention of hel" of the proposed response than on the "scientific rigor" of the answer.
But we must also understand that many of the novel researchers who access Researchgate seek in this platform, practical help to simple or common problems.
The numbers and ratings in this or any other platform are not a reliable indicator of the "professional level of expertise" and this happens in all areas, it is not the same to have 100 scientific publications in magazines of low-medium impact factor compared to 10 works In high-impact journals, however, in our "world" the number is often valued more than quality.
For example, the RG score gives a lot of value to the "interaction" between scientists and not only to the quality of the published works.
It may seem unethical, that novice researchers receive more recommendations than very expert researchers, but I believe that those of us who participate in this platform with some answers do not do so to receive recommendations (at least that is my case), but to transmit our experience (much or little) and knowledge (few or many) to those who start or do not have the means to solve their doubts.
Kind regards
What is the definition of expert? I feel, we all are same and here to exchange our views on the same scientific issues. I am one with your opinion prof. C. Serra-Rodríguez.
x-ray light scattering will be the best choice if you not prefered to use electron microscopy.
regards
vinous
Microscopy is the best option if you want your work to be published Dr. Sabry. Other methods like XRD, DLS can give some idea about the size. DLS can give the hydrodynamic diameter and the scattering methods have limitations (spherical) on the shape of the particles.
For imaging, sizing methods, etc. see::" Characterization techniques for nanoparticles: comparison and complementarity upon studying nanoparticle properties"
Article Characterization Techniques for Nanoparticles: Comparison an...
Thanks Mohammed Mothanna Numaan , Agnishwar Girigoswami , Vinous Hameed , John Clarkson and Hadeel Al - Nuaimi
There are one method, but not accurtae.
Scan your collidal good solution from 500 to 600 nm.
Usually at 520 nm pick is 20 nm particle.
526 nm pick is 40 nm
530 nm pick is 60 nm.
Above is rogh idea you have to check.
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