How to check the pH of E. coli culture initially? and how to change the pH of the E. coli culture?

I am working on E. coli 15597 prepared in Luria broth. Initially, the pH of LB is 7 after the inoculation of the E. coli it changes to 8.26 is it correct? can bacteria be alkaline?

15 October 2020 2,042 2 View

What are the best pipleline available for the RNA-Seq data analysis ?

What is the latest pipeline to run the RNA-Seq genomic data ?

12 October 2020 7,618 2 View

If an article is unknowingly published in a fake journal, is it considered as published or can the article be sent to some other journal to publish?

How do we handle this menace of fake journals?

14 July 2020 4,835 7 View

How do we differentiate conservation agriculture and regenerative agriculture in context of sustainable farming practice?

Sustainable farming practices appears into different names confusing the global audience which to follow and why? What makes conservation agriculture as different approach than regenerative...

30 June 2020 7,726 2 View

How to get DOI for my published article in a journal which has not given any DOI?

I want to know if Research Gate generates DOI for articles which has been published but has not been provided any DOI by the publisher?

23 May 2020 510 7 View

How many times do we have to check and report model fit measures during SEM?

I am trying to run a SEM which has four latent variables in it. Let us consider these variables as A, B, C and D. All are latent. Here A and B are independent variables, C is mediator and E is...

17 May 2020 4,091 3 View

What is the criteria to calculat e the plagarism?

What is the criteria to calculat plagarism for writing manuscript

06 April 2020 6,936 3 View

What happens if we lower the number of cycles in explicit dynamics for ansys ?

Hi I am a student pursing my final year in mechanical engineering. I am doing crash analysis of the car frame in Ansys software in explicit dynamics module. I had my analysis running for 2-3 days...

18 March 2020 3,709 3 View

Why contaminant is transporting from lower part to higher part in various column experiments? What is reason behind this?

can we transport the contaminant from the higher part to the lower part of the column?

25 December 2019 7,124 3 View

Can authentication in IoT be implemented using BlockChain techniques?

Authentication in IoT systems are vastly insecure. Mostly the problems lies on its open ended and probable untrustworthy nodes. Also, the need for a lighter authentication mechanism will always be...

09 December 2019 4,896 4 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

The procedure compute?

02 March 2021 5,204 3 View