I want to know how to make sephadex G50 column when you have Sephadex powder for PS isolation?

More Shubham Mathur's questions See All

How to determine tannin content in fodder pellets?

22 July 2024 8,163 3 View

Can anybody suggest freely available tools for Density Function Theory (DFT) analysis?

I need to study interaction between a metal ion and a multiatomic compound using DFT.

31 May 2024 9,532 4 View

Does anyone know how to Coat polystyrene particles with APTES ?

I want to have amine group attached on the polystyrene particle surface. APTES can be used to do that.

21 May 2024 8,092 0 View

How do I obtain sturdy hydrogel that doesn't dissolve in water upon centrifugation?

I have prepared cellulose nanofibers (CNFs) using TEMPO oxidation method. After sonication, the thin, heterogenous suspension turns into a thick, homogenous gel-like solution. Upon first...

13 May 2024 2,413 1 View

Literature review on consumer Behaviour, focusing specifically on Generation Z and their shopping habits in india?

literature review on consumer Behaviour, focusing specifically on Generation Z and their shopping habits in india

08 May 2024 4,593 2 View

What are the ethical implications of biometrics in light of the ADHAAR project of India?

Ethics

15 April 2024 6,778 1 View

How to use Blinder Oaxaca Decomposition Method ?

How to apply blinder Oaxaca decomposition methods to decompose the impact of two or more factors on a dependent variable on different Social groups?

28 March 2024 7,288 1 View

What are the benefits of function overloading in C++? Are the benefits ever been measured?

In C++, many functions can have the same name, which is function overloading. It works well but I don't find any benefits. Kindly educate me on the benefits of this overloading. Thanks

19 February 2024 6,745 3 View

How to make Google Scholar profile Publicly available ?

Hi, I'm new to this space, got some of journals published few months ago on nature, microscopy, both are open access. I checked my url is verified, Checked make my profile public selected Still...

15 December 2023 5,376 4 View

Which software is good to conduct a real-time Delphi for a research project on higher education?

I have planned to conduct a real-time Delphi survey that would involve Business school Deans. I am exploring online software that can assist me in data collection and analysis.

19 November 2023 9,223 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View