Rana Kamei

4 Questions 5 Answers 0 Followers

Questions related from Rana Kamei

What is ORF (Open Reading Frame) and Exon? What is their similarity and difference?

Seeking clearcut explanation regarding ORF(Open reading frame) and Exon. Some of the authors defination are complication. Like some says ORF is a stretch of DNA having Start codon at one and stop...

11 October 2022 3,568 4 View

How to extract peaklists from mass spectrometry raw file and do mascot search for my protein in ncbi?

I have a raw file of tandem mass spectrometry (LC-MS/MS) data. I want do online protein databank search for my protein identification and its homology.

31 January 2021 3,374 3 View

Can anybody explain a simple steps on determining the binding capacity of lactosyl-sepharose affinity column with protein sample?

I want to check the maximum holding capacity of affinity column against loading sample.

02 June 2019 4,242 1 View

How to activate sephadex G-50 for affinity chromatography?

What is the general protocol to activate sephadex G-50 for affiinity chromatography of lecitn?

15 May 2018 4,191 5 View