I wish to induce insulin resistance
Your best option is to carryout pilot study, because different strains and species react different in different geographical condition.
I am a second-year grad student. I am finding it difficult finding time to read literature and write (things like qualifying exam, thesis, grants). I find myself so busy with bench work that I...
01 March 2021 3,325 4 View
I've noticed that when thawing long-frozen samples (3-4 months at -20 C), most of the SARS-CoV-2 virus does not remain intact, but that the surrogate appears to. Has anyone else noticed this?
09 January 2021 4,168 7 View
I am woking with very low concentrations of RNA in an attempt to run a qPCR analysis. Given this, I am going to attempt to synthesize cDNA without diluting my sample, and in two different,...
12 December 2020 3,247 3 View
I am not a botanist but need to estimate the average percent vegetation cover of my study area. I have researched this and found articles that use the "releve" method for determining plot size...
22 September 2020 5,676 6 View
I'm running simulations for a bead-spring polymer networks using LAMMPS. I need my simulations to reach biologically relevant timescales of at least a few milliseconds. However each simulation...
14 September 2020 2,954 1 View
Is there such a thing as convex optimization for large deep learning networks? Is there a way to guarantee convergence? Has anyone looked into this?
06 August 2020 5,384 4 View
I am looking for empirical studies that examine the use of non-traditional grading in higher education. This means NOT using A-F grades during a course (even if, at the conclusion of the course,...
09 June 2020 1,519 6 View
- Ask questions where they are not sure/convinced about a statement/conclusion VS reject a statement/conclusion where they 'think' it is not correct? - Provide suggestions to improve a...
21 May 2020 2,624 7 View
On the side, I doing some research (literature-based) on a particular treatment that may help for COVID-19 symptoms but I need someone with a stronger medical knowledge base with expertise in...
29 April 2020 8,098 11 View
Hi , Im wondering does anyone know of any papers detailing the effects to training intensity of small sided games when the group is of mixed performance levels i.e( professional& semi...
18 December 2019 6,495 1 View
03 March 2021 8,272 1 View
Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...
01 March 2021 2,027 2 View
Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...
27 February 2021 3,904 2 View
Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...
24 February 2021 9,902 5 View
Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...
23 February 2021 8,155 5 View
Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...
22 February 2021 4,032 4 View
What are the testing rate for electrodes recommended by ASTM for cochlear implants. I am currently doing the 15mm/min elongatio testing.
22 February 2021 1,515 1 View
In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...
21 February 2021 1,864 2 View
Most of the data sets on the network only contain good information about the voltage and internal resistance of lithium batteries. The data of faulty battery is missing. Who can help me, I will be...
21 February 2021 8,659 1 View
Hi, all I am using some protocol for protoplast production from Fusarium graminearum (Fg), but I need them in a huge amount. Sometimes the protocol worked well but most of the time failed....
14 February 2021 2,286 4 View