I wish to induce insulin resistance
Your best option is to carryout pilot study, because different strains and species react different in different geographical condition.
I cut 50 um sections of tissue in agarose and need to culture them for 14 days before fixing.The sections have great surface area when I first section them, but after 14 days they shrink to sizes...
28 May 2024 9,861 0 View
I am preparing a protocol for SEM of a biofilm, but do not have easy access to 100% ethanol for the dehydration steps. Can 100% ethanol be substituted for 100% alcohol (95% EtOH, 5%...
07 May 2024 1,515 3 View
I was curious if anyone has used AI or other software for identifying a species (particularly a crustacean or crab) automatically? I am reviewing monitoring approaches for the European green crab,...
18 April 2024 7,408 2 View
I am looking to get a microscope for the identification of larvae in aquatic invasive species work. Would like to use it for the identification of larvae for aquatic invasive species work....
18 April 2024 4,401 2 View
Hello,o o o o o o o o o I have master's level training in logic and meta-logic, high marks, A's, but am not a practicing logician at all, I am a neo-empiricist pluralist (epistemic and...
18 April 2024 9,669 4 View
Hello! I need some guidance or clarification regarding the units for precipitation rate or precipitation intensity (esp. for rainfall). I'm used to precipitation intensity being expressed in...
26 October 2023 9,704 2 View
I want to ask you if this is a foram or not. If it is, what genre or even specie we're talking about? Please. This is a sample collected in the East Pacific Ocean, specifically in the coast of...
18 September 2023 6,667 4 View
Hello, I am currently doing a project requiring Camplyobacter lari growth. Our primary streak on Campy agar (similar to blood agar) produces very small (1 mm), translucent colonies, but after we...
21 August 2023 4,745 2 View
A previous colleague homogenized brain samples and stored the supernatant at -80C. According to their lab notes, the homogenized samples were centrifuged at 13,000rpm, for 15 minutes at 4C. The...
30 July 2023 2,992 0 View
Hi I am trying to make a set of cells that are associated to one or more faces and save them in a set using python scripting for Abaqus CAE, but I cannot make it work. I have created and example...
22 June 2023 9,548 1 View
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05 August 2024 4,407 0 View
I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...
01 August 2024 7,199 1 View
Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...
25 July 2024 2,532 3 View
I have done the same procedure with BL21 and pLysE but i got colonies but multiple times on rosetta i did but not getting. can anyone explain what would have gone wrong?
12 July 2024 9,904 1 View
Les géopolymères offrent plusieurs propriétés intéressantes qui peuvent être exploitées dans diverses applications innovantes : 1. Construction durable : - Bétons à faible empreinte carbone -...
10 July 2024 7,457 1 View
Continuous glucose monitors (CGMs) help patients and doctors make more timely management decisions by monitoring blood glucose levels in real time. An insulin pump provides continuous insulin...
07 July 2024 7,742 0 View
I have extracted essential oil from a medicinal plant and want to make several concentrations for some biological activities (Antidiabetic assay). How can i prepare the several concentrations to...
04 July 2024 8,100 4 View
If we want to introduce a material similar to polymethyl methacrylate "with the characteristics mentioned in the next paragraph" that can be added to a precursor and create a composite, which...
03 July 2024 2,371 1 View
We are fabricating a tandem structure where a ~50 nm thick WO3 film is deposited between two metal films using DC sputtering. We use a mixture of Ar and O2 gases at different flow rates while...
01 July 2024 2,686 2 View
thanks
25 June 2024 4,667 0 View