thank you for your reply. All the strains were isolated on MRS medium and plate on MRS agar. I want to identify the strains out of my country. Can someone help me?
I think best way i would recomend for you is to go for metagenomic analyis, isolation is not a better technique, I have used metagenomic analysis on fermented milk products and feel it would be the best suited for you as they can provide you the approximate estaimation of the population.
I had taken help from Dr. Scott
http://scotedowd.com/
if you are interested you can contact him and he has also authored several articles in the feild of metagenomic analyis