i want to find the virulence factor and common gene both raw data is present here plz f some one already done plz help me
What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?
28 February 2021 6,516 5 View
I want to publish my pilot study about corrosion in RC structures in any journal. Please help me to find good options.
25 January 2021 6,413 3 View
Soil fertility, plant nutrition
23 December 2020 7,301 5 View
In ROMP M:C is 100:1, so whats the ratio of Quencher be added in? will quencher precipitate the polymer?
01 December 2020 1,283 4 View
I am looking for print/tv/social media/outdoor anti-counterfeiting advertisements targeted to British consumers.
23 November 2020 7,950 2 View
Hi all I would like to study the microstructure evolution of semi-solid tin-lead alloy for an extruded sample during the isothermal heat-treatment process. Is there any difference if I heat up...
18 November 2020 9,087 7 View
I wanna simulate graphene-based polymer composite material for synthesis and testing of their different properties.
15 November 2020 6,040 5 View
Dear Friends, I would like to know the freely accessible software name for making the graphical abstract and illustrated diagrams for research publications. Sharing the link will be useful to...
30 October 2020 623 19 View
Hi, I was reading the paper (MicroRNA Expression, Survival, and Response to Interferon in... ). In Table 1, I'm wondering how did the authors calculate a p-value of 0.29 for Age? The age in Table...
20 October 2020 2,276 5 View
Any close value will also work.
03 October 2020 8,067 4 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
Hi all, I stained tdTomato+ cells (PBMCs and Jurkats) with eBioscience Foxp3 / Transcription Factor Staining Buffer Set for the foxp3 and I completely lost the signal of tdTomato. I tried to fix...
10 February 2021 5,863 2 View
I am working with a very unstable transcriptional factor. I use DNAse in the lysis buffer and I purify it as inclusion bodies, by using urea and 1M NaCl. After purification, I buffer exchange the...
05 February 2021 2,530 3 View
Hi everyone, I would greatly appreciate it if someone can shed light on certain endothelial functional assays and their conditions. I often notice that protocols are run with serum-free media, or...
05 February 2021 7,276 1 View
My question is regarding a western blot experiment that I am performing to observe pERK signaling by stimulation with VEGF ligand (VEGF165 and VEGF121) on MDAMB231 cells and SW620 cells (both...
30 January 2021 1,039 3 View
According to information from online database [MRTFA - Myocardin-related transcription factor A - Homo sapiens (Human) - MRTFA gene & protein](https://www.uniprot.org/uniprot/Q969V6) and [MKL1...
12 January 2021 9,492 3 View
I have to control the expression of a gene ( in vitro ). For designing an optimal promoter assay, I would like to know more about Transcription factor binding region, enhancer and repressor...
11 January 2021 7,992 3 View
NEW COVID_19 Strain_description, virulence
01 January 2021 9,620 6 View
I really need help. I tried finding a protocol on how to prepare samples for gene expression . I am working on Antimicrobial activity of a medicinal plant and I would like to check its effect on...
30 December 2020 7,112 2 View
I am looking for recommendations for good resources or guidelines for developing surveys specifically focused on targeting user's opinions or a needs-analysis in the context of engineering design...
12 December 2020 6,698 6 View