I am planning to use tumor cell lines from alveolar Rhabdomyosarcoma.
As a stable element
23 July 2024 4,187 1 View
Hello, dear RG community. Personally, I have found Xarray to be excruciatingly slow, especially for big datasets and nonstandard operations (like a custom filtering function). The only suggestion...
15 July 2024 4,705 2 View
o impacto da Gestão Estratégica de Marketing, na tomada de decisões empresariais?
10 July 2024 3,430 0 View
Hi all, I am working on both primary hippocampal mouse neurons and i3Neurons to isolate axonal material. I plate them in microfluidic devices in house made (like any other you can find on the...
01 July 2024 8,070 0 View
Can you use the MTT assay of cell viability for tissue samples derived from rats e.g. liver, muscle, heart, brain, or is this method reserved for cultured cells? Thank you
29 May 2024 6,389 2 View
Hello everyone, I am currently exploring several options to give the collected data the greatest value possible. I have demographic data on older people, where I perform various memory and mood...
02 May 2024 876 6 View
Could you please provide me some authoritetic refers where this formula used if it accurate OR tell me why it is wrong and how to fix it? 1. TOA (L) = ML * Qcal + AL 2. BT = (K2 / (ln (K1 / L) +...
19 April 2024 8,924 0 View
Hello, dear RG community. Very often, gas fraction (also known as void fraction or gas holdup) is thought of as a ratio of the gas volume to the total volume:...
04 April 2024 7,290 0 View
Dear Researchers, I am reaching out to this esteemed community seeking insights into a recent experiment I conducted involving cell seeding in agarose micro-wells. My aim is to engage with fellow...
22 March 2024 4,438 0 View
I'm researching about videogames and their effect on metacognition, actually I intend to develop a game with that specific intention. I'm tinkering with a puzzle game that gives feedback and maybe...
18 March 2024 1,147 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
