I want the Early-diverging fungi (e.g., Chytridiomycota, etc.) cryopreserved at -80°C, but I do not know what is the concentration of glycerol used?

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Yehya A. Salih

Dear Jun-En Huang,

Greetings and have a nice day.

You can use 10% Glycerol in micro tubes (2 ml) and preserve them under deep freezing (-80 C) for a long period.

With regards.

Jun-En Huang

thank you, Yehya A. Salih

Nazia Manzar

10% glycerol is best for fungal preservation and 20% glycerol is best for bacterial preservation.