I used three primers Bcsp31(B4,B5), IS711 & B.abortus & IS7711 & B.Melitensis for the control positive and I got nice result with samples I got smear?

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The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

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27 February 2021 9,356 3 View

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What is the most suitable medium of ascomycetes fungi ??

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23 February 2021 3,840 3 View

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What is the ideal efficiency for a qPCR?

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Robert Adolf Brinzer

Are these individual colonies you are testing or are you using mixed samples that can contain other bacteria as well?

Kaadhia Al-Kharousi

NO, it DNA extracted from milk and buffy coat blood. only I am testing to see type of Brucella.

Then could be there is off target amplification of other bacteria species in the sample.