Hi, Here I want to culture the mice feces bacteria, is there any medium fit for the bacteria from feces to grow? I mean how to culture the fecal bacteria as much as possible? Thanks!
For your 'if you use a highly nutritive bacteria (hoping to cultivate as much bacteria as possible) you may loose some very important genes on plasmids present in many bacteria'. do you have reference regarding this conclusion? Thanks!
I know I can cultivate very limitted amount of bacteria, but I want to cultivate as much as possible, so this kind of medium is very important, to be specific for mice fecal bacteria.
If you wanted to analyse the bacterial content you would be better to carry out either a 16S or Whole metagenomic sequencing of the feces samples. As the others suggest, if you culture you will only see about 1% of the bacteria that are present. The other 99% will not be able to be cultured.