This is the used protocol:

•Spin down an aliquot of Amyloid 42 HFIP** (~ 5 mg/ml), then 1 µL of Amyloid 42 was diluted in 20 µL PBS.

•5 µL of 4x Laemmli protein sample buffer for SDS-PAGE was added. The sample was boiled to 95 degrees for 5 minutes. Then, short spin down.

•Then, the sample was run along the ladder (BLUeye PrestainedProtein Ladder) in 4–20% precast polyacrylamide gel, 8.6 × 6.7 cm (W × L), for use with Mini-PROTEAN Electrophoresis Cells.

•The gel was stained with Quick Coomassie Stain for 1 hr.

•Lastly, the gel was scanned.