Hana Marz

5 Questions 6 Answers 0 Followers

Questions related from Hana Marz

How can we improve the sonication process to achieve homogenous fibrils of the desired length (<= 50 nm) as indicated by Dynamic Light Scattering?

We conducted Dynamic Light Scattering (DLS) before and after sonication of our preformed fibrils of alpha-synuclein (aSYN) using a microtip sonicator. Our protocol was as follows: Amplitude:...

11 December 2023 2,749 1 View

Taking IF images using Evos M5000 FL Microscope?

Hi, I'm trying to take images for my fixed coverslips with cells. However, when I mount them using a mounting medium with DAPI, I try to take focused images. Few slides are better than the others...

15 September 2023 9,461 0 View

I run an amyloid 42 sample on SDS-PAGE but I did not have a band. Any thoughts why?

This is the used protocol: •Spin down an aliquot of Amyloid 42 HFIP** (~ 5 mg/ml), then 1 µL of Amyloid 42 was diluted in 20 µL PBS. •5 µL of 4x Laemmli protein sample buffer for SDS-PAGE was...

23 July 2023 7,637 1 View

I run a IF staining on cultured cells. In one case, I did not apply the primary antibody to test it. It gave me signal. Why?

How can I reduce the non-specific binding? I applied a 5% Blocking buffer. Can I use 10%?

23 July 2023 1,320 2 View

How can I interpret the fluorescent readings taken using a 96-well plate and a Plate reader with a dual staining Acridine Orange and Propidium Iodide?

As you are aware, Acridine orange is an intercalating dye that can permeate both live and dead cells. AO will stain all nucleated cells to generate green fluorescence. Propidium iodide can only...

14 June 2023 7,590 9 View