Electroporation could be used. Does anybody have an experience in electroporation with proteins or peptides? It would be very helpful if you share it. The second solution is streptolysin. Maybe there are any better methods?
I have attached a pdf for a study of electroporation of peptides into cells. Electroporation is one of those things that is highly cell type dependent, and needs to be optimised for each cell line. The buffer and pulse protocol will affect permeation as well as cell viability. If you are using an Amaxa electroporator, there are optimisation kits you can get to maximize permeation and viability.
Your other choice would be to optimise a liposome based method to get the peptide into cells or add a poly-Arg (11R) sequence to your peptide, both of which could be useful if properly controlled and optimised.
For proteins the following kit works....i dont know about peptides. But it may help you...
http://www.piercenet.com/browse.cfm?fldID=32CD01C1-F439-4145-B0C5-D1BC7DA66922
I'm not sure what your sequence is, but if it is hydrophobic, you can use albumin as a carrier protein. Albumins are "sponges" for hydrophobic molecules. Electroporation of your peptide in the presence of BSA may increase your penetration.
Thank you for useful advises and references! The peptide is unfortunately not hydrophobic but polyR is a good variant for a plan B )
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Cell Biology
- Culture Cells
- Cell Line