Valentina Bozok

7 Questions 17 Answers 0 Followers

Questions related from Valentina Bozok

May pinocytosis of peptides improve the viability of cells?

I work with Jurkat cells. A relatively high concentration (up to 500 uM) of small peptide (4 aa) is added to growth medium and pinocytosed by cells. The peptide shouldn't have any effect, but it...

02 February 2017 8,853 4 View

How to transfect Jurkat efficiently?

I need to transfect plasmid DNA into Jurkat cells, but it seems that these cells are hard to transfect. If anyone has an experience, can you share it with me?

10 October 2014 437 10 View

Is it correct to calculate statistics comparing to the same experimental group to which values were normalized (SD=0)?

one-way ANOVA with Dunnett’s post-test

10 October 2014 3,846 9 View

Highly expressed protein in Jurkat cells?

Here is a SDS PAGE of Jurkat total lysate. Can anyone suggest what is the highly expressed protein? Unfortunately a marker is absent.

08 August 2013 2,340 8 View

Can someone advise on calcein AM stains apoptotic bodies?

Calcein AM should stain only living cells but it stains apoptotic bodies as well. What may be the reason? I use Jurkat cells

07 July 2013 8,024 8 View

I need to put a small peptide (4 aa) into the cells (mammalian lines).

Electroporation could be used. Does anybody have an experience in electroporation with proteins or peptides? It would be very helpful if you share it. The second solution is streptolysin. Maybe...

04 April 2013 4,878 4 View

Can insoluble protein from inclusion bodies solubilize if its concentration decreases (during dilution)?

I have separated soluble and insoluble fractions of two proteins that differ by some amino acids. On the picture 1 - total, 2 - insoluble and 3 - soluble fractions of one protein. 4, 5 and 6 refer...

05 May 2012 5,426 5 View