Does anyone have experience with phospholipase C mediated release of GPI anchored proteins on the membrane?

I am expressing a recombinant mcherry red protein by using a pVAC1-mcs vector in HEK293 cells. pVAC1-mcs has a 3' glycosylphosphatidylinositol (GPI) anchoring domain of placental alkaline...

04 April 2013 9,355 1 View