I need this primer for ER stress assays.
does the rat genome database http://rgd.mcw.edu/ help. It should have the gene sequence to design your primers
Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance
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Dear Scienstists Why secondary peak is found in PL spectroscopy? Does this have a physical equivalent or meaning? Can anyone who knows please answer me personally?
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because when simulated was s11=-0.0012 dB at 3.318 GHz when connected with my filter design was excellent s11 greater than -90 even it has bad s11 THE problem is there is different result when...
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I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
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I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
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After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
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Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks
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i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
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I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
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How to design VN primer to attach with universal reverse primer
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I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
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I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
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