I need pairs of primer for quantification of Lactic-acid bacteria (all species), E-Coli (all sub species) and gram-negative bacteria by Q-PCR?

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Please introduce AI website for paper review like Chat GPT?

01 March 2024 3,173 5 View

What form of silica and particle size are suitable for fostering the growth of Diatom microalgae?

What form of silica and particle size are suitable for fostering the growth of Diatom microalgae? Can nano-silica be employed in the context of promoting Diatom growth?

19 December 2023 964 3 View

Kinematic transformation of CA spindle-tilting machine?

Hi dear colleagues, I failed to find the transformation matrix from the 3-axis (workpiece coordinate) to the 5-axis XYZAC (machine coordinate) table-tilting setup. Does anyone know any source?

07 November 2023 6,284 0 View

Clarification on "Space of Economy" vs "Place of Economy" - Seeking Insights from Scientific Articles?

Dear Researchers, I hope this message finds you well. I am currently delving into the concepts of "space of economy" and "place of economy," particularly in the context of economic geography,...

19 August 2023 3,196 2 View

What inflammatory biomarkers should be measured in individuals with non-alcoholic fatty liver disease(NAFLD)?

There are many biomarkers such as TNf-Alpha, CRP, and IL-1, but I'm looking for some novel biomarkers to measure.

21 March 2023 5,346 2 View

How can I disperse C3N4 nanosheets?

After sonicating, my solution precipitated, pleased help me.

13 August 2022 7,313 0 View

How do we calculate the sublimation energy in f titanium in vacuum?

I was trying to calculate the sublimation energy but of Ti in 10^-8 torr. I understand that sublimation energy equals: E(delta t in solid)+E(solid to liquid)+E(delta t in liquid)+E(liquid to...

20 November 2021 1,169 3 View

What happens if the autocorrelation is considered incorrect in volatility estimation?

In time series modeling and volatility estimation it is necessary, first remove autocorrelation of time series and after that estimate the volatility model (like GARCH). the autocorrelation...

02 October 2021 2,488 4 View

What are the required material properties to perform fatigue simulation using ANSYS SMART?

Hello dear colleagues, I couldn't figure out what are the required material properties to perform fatigue simulation using ANSYS SMART. What should I add to predefined structural steel to proceed...

05 September 2021 4,647 2 View

Reaxff MD simulation?

who can help me to learn this method of molecular dynamic simulation or will it provide me with educational material?

01 September 2021 9,098 7 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

Z-average size from Dynamic Light scattering can it be lower than the original probe size?

Hi everyone, Recently I have been conducting Dynamic Light scattering experiments in a micellar solution at 5 and gel at 37 degrees of Celsius with latex particles of diameter 190-500 nm. While...

01 August 2024 1,168 4 View