I'm looking for different RCA's classifications in America?

Please help me find a brief history of Recycled Concrete Aggregate (RCA) CLASSIFICATION in the U.S. any paper or Official U.S. state DOTs' document. Target: To find out how many classifications of...

12 January 2021 7,564 3 View

How can I insert an assembeled system, meshed by SOLIDWORKS, to ANSYS?

I already did it before but this time as the model is complicated I have faced with tis error: The surface mesh is intersecting or close to intersecting,making it difficult to create a volume...

05 September 2020 9,558 1 View

Is there a way to calculate energies for all residues from a pdb?

CHARMM GUI online does a protein-protein interaction where you can measure the interaction energy of one chain with all the others. Is there a way to do this for each residue? Basically I want to...

29 July 2020 8,851 3 View

Role of intensity in UV-Vis Spectroscopy?

I have two suspensions of Gold Nanorods in Deionized Water synthesized by the same experimental route. Each of the GNR solutions shows the same peak but with different intensities. Is this related...

14 July 2020 9,680 0 View

Why would we see generated cracks during heat treatment of the additive manufactured Inconel 625 ?

18 June 2020 2,618 5 View

Mechanical and Physical test Properties of Concrete Aggregate?

Is there any specific reference such as papers or books which have introduced Concrete aggregates tests under Mechanical and Physical Properties? For instance, specific gravity is a Physical test...

10 June 2020 8,611 19 View

I want to compare the difference of immune system in patient with mild and sever symptom.who should i define the case and control group?

Is it enough to compare people who needed to be hospitalized with people who did not? , or should the age and duration of the illness be considered?

26 May 2020 2,829 2 View

how can i removed the tackiness of polyborosiloxane (poly borodimethylsiloxane)?

I have synthesized a PBS. this material have a tackiness after synthesized and stick the aluminum foil. how can i removed the tackiness of polyborosiloxane (poly borodimethylsiloxane)?

23 April 2020 2,161 3 View

Why the covid-virus infection is very rare in children compare to young and adult people?

As we know the immune system in both children and adult is impaired. The question is that why the covid-virus infection is very rare in children compare to young and adult people

03 April 2020 9,934 11 View

How to prevent the elongation of the generalized beam section in the Abaqus Static-General or Dynamic-Explicit Analysis?

Dear All, I modeled a rotating blade in the Abaqus/CAE 6.14-2 x64. The blade is generated using the properties of different sections of the airfoils along the span. The generalized beam section is...

17 March 2020 3,400 4 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View