How Triton-x removes SDS from SDS-Polyacrylamide gel?

I came to know about removal of SDS from Polyacrylamide gel is done by Triton-X, but what is the exact mechanism of its removal. How Triton-x does that? Thank you

03 February 2021 7,206 2 View

Typical concentrations of glucose transporters in mouse brain?

I have measured levels of GLUT1, GLUT3, and GLUT4 in the mouse hippocampus and cortex using ELISA kits. I'm trying to find typical concentrations (in ng/mg total protein) of these proteins in the...

21 December 2020 4,647 2 View

How can we differentiate crystalline peak to an amorphous peak of XRD ?

When characterized with XRD , my prepared sample show peaks which are broader(bottom) than crystalline peaks but unlike amorphous materials, have sharp top. Is there any limit of intensity or full...

15 December 2020 671 4 View

How Shall I perform Principle Component Analysis in GROMACS?

I have to perform PCA as a part of my results and analysis of protein-ligand complex docking using GROMACS 2018.8. How shall I perform PCA and interpret the results for the same? Any insights...

13 December 2020 3,121 2 View

How shall I prepare a protein PDB file in a particular pH for docking and simulation?

I wanted to prepare a protein at an alkaline pH for molecular docking as that is the pH I want to perform my in vitro assays in? I prepared my protein at the required pH through H++ server;...

13 December 2020 2,272 3 View

Can enzyme that is extracted from Aspergillus niger be used in the range of 4-40% v/v for optimization of veggie juices like broccoli??

09 December 2020 7,083 3 View

How to identify GC peaks for FAME mix which do not match reference peaks?

Hello, I am analyzing 37 component FAME mix using GC. I have got 37 peaks and 25 of them show expected order and height. However, others are not in the similar order with the reference for same...

22 November 2020 7,033 4 View

What could be the many interpretations of the Bass Model?

The Bass model has drawn a lot of research over the years and subsequent research has interpreted the model in numerous ways like Lekhvall and Kahlani. I would love to know any other ways the...

03 November 2020 6,434 1 View

Regarding FAR, FRR, EER curves?

I have the image database, but I need to calculate the FAR, FRR, and EER and plot them. I am unable to understand how to plot them as they are the images... I am unable to make a program using the...

05 October 2020 3,459 3 View

Which of the five PDB files (DRGFIN.PDB, DRGMMX.PDB, DRGNOH.PDB, DRGPOH.PDB & DRGAPH.PDB) as an output from PRODRG Server do I need to use?

I have some protein-ligand complex to simulate on GROMACS version:unchecked. For preparation of my ligands, I submitted the extracted Ligand.pdb file and submitted it in the Prodrg server, Energy...

01 September 2020 9,154 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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28 February 2021 9,936 2 View

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23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

What is the actual chemistry going on between iodate and ascorbic acid?

I am studying biochemistry at university, as part of the course we do several titration experiments. In our handouts, we were given the following half equations: IO3- + 6H+ + 4e- I+ +...

16 February 2021 8,731 1 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

Any probe or assay for the detection of Cysteine in yeast invivo ?

Dear fellow researchers, I would like to detect the cysteine levels for yeast in vivo. I would really appreciate if anyone could suggest me any fluorescent probe or assay kit available. Currently...

11 February 2021 3,292 3 View

How to assing secondary structure to calculated peptide/protein having xyz file?

Hi, I have an optimized structure of some peptides and I would like to represent it as a secondary structure - cartoon. Ok, so server apps ask for pdb file (like Stride). I tried in different...

04 February 2021 517 3 View