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Questions related from Swati Kundu
Hi, I want to attach biotin-lysine at the C terminal of peptide sequence [DPVLWRHFILSGPGYK(Biotin)CONH2]. I read that 20% piperidine can be used for deprotection (Solid-phase peptide synthesizer)...
24 November 2021 2,344 5 View
Hi, I am synthesizing pentapeptide (GGGYK-Biotin) by FMOC SPPS using Rink amide resin. GGGY is a linker and K-biotin is a label. Unlike other fmoc amino acids, biotin-lysine did not get dissolve...
11 November 2021 1,140 4 View
Do we get a trifluoroacetic acid (TFA) peak during gel filtration (desalting) of the synthesised peptide after RP-HPLC purification (0.1% TFA in buffers)? The molecular weight of my peptide is...
12 June 2021 8,233 2 View
Hey! I need help on peptide (22 aa) oxidation.This peptide has no methionine, 1 tryptophan & 1 tyrosine. also has 4 cysteine so I used 2 protection groups (trt & acm). For ACM removal, I...
04 December 2020 3,380 2 View
