.
If you have FACS if not RT-PCR of cyclines specified for each step of cell cycle
If you have FACS do PI staining if not RT=PCR for cyclines and CDKIs
thank you.. in case of RT-PCR of cyclines ..you mean analysing the checking points in which the cells pass from one phase to another ? and if so ..threre will bw any specefic changes?
Yes, an increase and/or decrease in cyclines may indirectly suggest to the cycle the cells are but the best method is FACS.
I am Intrested in molecular dynamic simulation for studying the interaction of several compounds with the cell membrane.
02 March 2018 4,505 1 View
I'm looking for a good model for brain melanoma metastasis to investigate in mice.
10 November 2013 6,875 18 View
I am trying to quantify doxorubicin accumulation in B16BL6 melanoma bearing mice. What is the right method for extracting the drug then quantifying it using flourescence measurment?
04 May 2013 4,601 6 View
I'm trying to study doxorubicin uptake by cancer cells using confocal microscopy. What would be the best filter to use and what is the best conditions concerning fixing and washing etc.?
04 May 2013 3,850 9 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...
02 March 2021 2,246 2 View
Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?
01 March 2021 8,087 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
28 February 2021 2,534 2 View
I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...
26 February 2021 10,041 3 View
I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...
25 February 2021 3,030 1 View
Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...
25 February 2021 3,443 4 View