I need a reliable recipe for pcr elisa buffers that are lab made and that have pbs not tbs?

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Christian Praetorius

Why don't you like TBS? Except for cases in which phosphate really is disturbing a reaction, it makes basically no difference. Both buffers work very good in controlling the pH.

Elaheh Hashemi

Because i have ready tablet pbs in lab

@Elaheh: Ok, this is an argument. But since neither PBS nor TBS are really difficult to make, it shouldn't matter.

Devendra Vaishnav

I would recommend to get the Book "Molecular Cloning: A Laboratory Manual" which will answer most of your questions.

http://www.molecularcloning.com/