Elaheh Hashemi

5 Questions 11 Answers 0 Followers

Questions related from Elaheh Hashemi

What is the probability of replacing U nucleotides with digoxigenin during PCR amplification?

Thank you.

02 February 2015 4,444 1 View

How do you decrease OD of negative control in PCR Elisa?

My PCR Elisa negative control has the same OD to positive near 1. I am using nunc streptavidin-coated plate and its protocol so it doesn't need blocking.

05 May 2014 3,464 1 View

Can anyone provide exact and reliable content of PCR elisa solution to make buffers?

I had a kit but my PCR elisa didn't answer so I want to make the buffer myself.

04 April 2014 9,453 11 View

Is tween20 require during washing steps in PCR ELISA?

In common ELISA for protein in washing steps we use pbs with tween but I want to know in PCR ELISA that we work with DNA for washing unbinding bond do we need tweeen or it has negative effect for...

04 April 2014 6,605 8 View

I need a reliable recipe for pcr elisa buffers that are lab made and that have pbs not tbs?

I want to do my pcr elisa with hand made buffers. I need a protocol but I prefer using pbs in washing not tbs.

04 April 2014 4,624 4 View