Devendra Vaishnav

11 Questions 21 Answers 0 Followers

Questions related from Devendra Vaishnav

How can I measure total phenol and total flavonoid in different extracts?

I have prepared crude extract from the plant material and then fraction it with different solvent. How can I calculate the total flavonoid and total phenol in each fractions. First I extract the...

02 February 2015 1,220 9 View

How does the Comet assay (Single cell electrophoresis) work?

I am working on plant extraction and I want to check the DNA protecting activity of my extracts. How does the comet assay work? Is there any other alternative to comet assay? Can anyone provide or...

04 April 2014 6,604 5 View

What is the basic principle for selecting mobile phase in preparative column chromatography?

I am trying to separate the phytochemicals from the crude extract. What parameters should I consider for the mobile phase selection for column chromatography?

01 January 2014 6,384 10 View

What is the normal range of insulin in rats and in STZ treated rats?

I am working on plant extracts active against type I diabetes. I would like to know the normal range of Insulin in normal rats and STZ treated rats.

12 December 2013 8,854 5 View

How can I remove chlorophyll from leaf extract without losing other secondary metabolites?

I am working on isolation, purification and characterization of biomolecules from leaf extracts. I have methanolic extract of leaf and I want to remove the chlorophyll from it first.

10 October 2013 7,685 15 View

How much concentration of primary and secondary antibody is required to perform the western blot?

I purchase primary PPARγ antibody (H-100) is a rabbit polyclonal IgG provided at 200 µg/ml and secondary goat anti-rabbit IgG-HRP: sc-2004 200 µg in 0.5 ml volume. How much I dilute to perform...

10 October 2013 2,500 4 View

How can I select the plant active against diabetes?

On what bases I can select the plant against diabetes? Is there any strategy for selecting plants for specific diseases.

10 October 2013 9,186 6 View

What is the basic difference and what are the applications of immunohistochemistry (ICH), immunoflorescence (IF) and immunoprecepitation (PI)?

I want to know the basic differences between these techniques and their application in molecular biology and pharmacology?

10 October 2013 8,222 1 View

Which method is best suited for estimation of Cox II in diabetic rats, ELISA or Western blotting?

Kindly help me to choose the appropriate method for estimation of Cox II in diabetic rats, ELISA or western blotting.

09 September 2013 4,061 20 View

Could anyone advise me on the process of purification of alkaloids from crude alkaloidal extract?

I have already collected the total alkaloidal extract from crude by acid base extraction. How can I isolate all the alkaloids present in total alkaloidal extract?

09 September 2013 3,550 7 View

How can I separate Alkaloids, Glycosides, Triterpenoids, Seponins, Flavonoids, etc. from crude extract?

How can I Separate the Alkaloids, glycosides, Triterpenoids, Seponins, Flavonoids from the crude extract without loosing any of the compound by single extraction procedure.

08 August 2013 4,302 20 View