Hi Hussam, just checking you do have an absorption spectrum? For nanoparticles, if you use the conventional dual beam system you would be measuring an extinction spectrum, which is a sum of absorption (A) and scattering (S). The transmission (assuming this is what you meant by transition) spectrum (T) would be calculated directly from the extinction spectrum (E), where T=10^-E. A and S can be separated by using something additional like an integrating sphere. I don't think you can get a reflection spectrum directly from the absorption spectrum, but I might be wrong.
If one has transmission and reflection spectrum, one can get absorption. However, if one has absorption data, it is not easy to separate into transmission and reflection.
I assume the missing part in the discussion is Diffuse Reflectance measurement which can be performed on powder samples.
Hi Hussam, I think you already have the extinction spectrum, gathering from what you have described.
I agree with Ryo Sekine that you already have what you need for calculating the transmittance spectrum. The small confusion originates from the term "absorption". Absorption A is defined as A=100-T-R-S, where T is transmittance, R is reflectance and S is scaterring. The parameter that you have already measured is absorbance (or extinction) (E) and it is equal to -log(T). Thus T = 10^(-E) as Ryo Sekine already suggested.
thank you for all
Origin file attached for absorption may you find another parameters T R
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