I have quantified beta-glucosidase activity by ONPG, what is the unit of activty in this method?

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Adam B Shapiro

A fairly standard definition of "unit" of activity in enzymology is the production of 1 micromole of product per minute. A set of standard conditions (buffer composition, temperature, substrate concentration) for the assay should be defined and reported.

Ajay Kumar Sharma

Thanks to Dr Marka and DrAdam.

Gunajit Goswami

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