I have problem with designing a primer for my gene,how can i fix it???

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Best novel method to optimize 3D geometry and its fluid?

Do you have any idea what is the best method to optimize 3D geometry and its fluid without linking the code with fluent (or any flow solver) ? In order to find the best 3d geometry and decrease...

10 April 2020 9,321 1 View

I have problem with cytotoxicity test of gemcitabine.how can i fix it?

Hi, i am working on effects of gemcitabine in HepG2 cell line and i should use the IC50 of Gemcitabine in the experiments.i found different values in literature and the numbers are highly variable...

06 December 2019 6,382 3 View

What is the IC50 of Gemcitabine in HepG2 cell line?

Hi, i am working on effects of gemcitabine in HepG2 cell line and i should use the IC50 of Gemcitabine in the experiments.i found different values in literature and the numbers are highly variable...

26 August 2019 8,545 4 View

In flow analysis in FLAC software, what is the reason for moving the model after the flow analysis in the earth dam?

Hello I have a model of earth dam in FLAC 2D and I did an uncoupled analysis for mechanical and flow. (set mech off/flow on ) Next step, when mech on/flow off ,the model starts to move in the...

14 July 2019 5,146 3 View

Do any researcher have ideas for the best medical Phantom that could be employed in segmating 3D medical data?

I am doing a research for segmenting 3D medical volumes using different techniques I used recently NEMA IEC body phantom and CIRS abdominal phantom,model 057 in my research I am looking for the...

19 May 2019 9,979 2 View

How can i clone a plasmid?

how can i clone a plasmid that i don't know any thing about its sequence , and the backbone of the plasmid?

05 January 2019 6,028 15 View

Is degenerate primers appropriate for real time pcr?

hi... can I use degenerate primers for real-time? I have some genes related to the enzyme in the root but these genes are gene families .and I don"t know which gene controlled my desire enzyme,...

20 December 2018 3,452 2 View

Why do i have band in negative control in ITS primer?

I look for variation in strain bacterium and I want to do sequencing but I have a band in negative control that same in my strain bacterium first time I noticed band in my negative control, I...

02 December 2018 5,889 10 View

The data of maximum specific growth rate in the continuous biorector versus pH?

Hello Dear all I want to examine the effect of pH on maximum specific growth rate in the continuous biorector for acetic acid production by Acetogenium kivui or Clostridium thermolacticum...

17 October 2017 9,311 1 View

How to use the Modulus of Elasticity of Sand in PLAXIS?

The modulus of elasticity differs in various conditions of foundation soil. I am struggling with defining modulus of elasticity of geosynthetic reinforced sand foundation beneath a shallow strip...

01 January 1970 5,965 4 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

Is stone a sustainable material? Is it a sustainable approach to use natural stone waste in architecture?

I'm designing a zero-waste restaurant and considering using marble waste to make some tables and a bar countertop. My clients think it's not "sustainable enough" and I would like to have more...

01 March 2021 4,326 7 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

Microstrip antenna design tutorial of HFSS 3D layout module ?

I have designed a 24 GHz rectangular antenna on HFSS 3D model and got desired results while desing the same antenna with same dimensions I am getting resonance frequency at 27 GHz I am...

28 February 2021 4,426 2 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View