I have problem with designing a primer for my gene,how can i fix it???

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How to estimate the best cell count for Single Cell RNAseq?

We are about to initiate #scRNAseq on some T-cell types. Back to literature, I found a wide diversity in the counts of the anlaysed cells used for the method (1K - 25K cells). I would really be...

13 February 2024 8,947 2 View

Is anyone currently using a programmable peristaltic pump with 4-roller pumping heads and 1/16" ID tubing from Braintree Scientific, Inc.?

I am in the process of setting up a programmable peristaltic pump for mouse cardiac perfusion. I'm uncertain about the appropriate blunt needle specifications, specifically 24 gauge (24g) with a...

12 November 2023 9,653 0 View

Overfitting- corrected calibration curve of Poisson regression model?

I am going to plot an overfitting- corrected calibration curve(using the cross-validation method) for a regression model with Poisson family (link function=log) in R. I know that this can be done...

24 July 2023 4,291 4 View

At what temperature should M100 tensile test be run for crosslinked EPDM Rubbers?

I would like to run M100 tensile test on beamed EPDM rubber samples per ASTM D412-06 (different beam conditions) to determine mechanical strength of crosslinked rubber. I know for...

21 February 2023 6,868 2 View

Tosylation glycerol carbonate?

I tried to tosylation glycerol carbonate with sodium hydride base in THF solvent. but C=O bond in glycerol carbonate was broken. can you help me what is the reason of that? is it becauce of the...

14 November 2022 1,795 4 View

Why PMMA thin film prepared by dissolution of PMMA in Chloroform is not transparent (it is opaque)?

I want to make a thin film from PMMA in CHCl3 solution with a 200 mg/ml concentration. The PMMA dissolved well in CHCl3 and it was optically clear and transparent, but the thin film was opaque. I...

27 October 2022 1,658 3 View

What is the relationship between phase stability and the amplitude dispersion across a time series of InSAR image acquisitions?

For a particular pixel across multiple co-registered InSAR images, the amplitude value of the received echo may fluctuate from a mean value based on how sustained/changing is the scattering...

21 August 2022 2,772 2 View

What is The best culture for Streptomyces pilosous?

Hello, Would you please let me know, What is the best culture medium to increase Streptomyces pilosus production?

07 June 2022 8,743 4 View

How to inject a certain number of particles in DPM model of fluent?

Hello everyone I am trying to simulate 1000 particles in a microchip. The adjustable parameters in the injection part of discrete phase model (transient) are flow rate(kg/s) and start/stop time....

24 January 2022 4,426 3 View

Is it possible to divide the time interval in a time-dependent study simulation in Comsol software?

Hello everyone I have a question about Comsol software. I use a time-dependent study and I want to divide my interval so that I see the results every five seconds and if they are correct, I give...

04 December 2021 2,165 3 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Does continues partial charging/discharging do any harm to the BESS performance and degradation?

For a grid connected BESS (lets say 100 MW/200 MWh system, LFP battery), if we charge the BESS in such a way that whenever we can access power from the solar that will charge the BESS. Hence it...

01 August 2024 9,903 7 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

Do you mix and add all primers together in a singel PCR reaction (4 total primers for 2 mutations) when using Agilent's multi-site mutagenesis kit?

I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...

25 July 2024 6,517 3 View

I am trying to find phonon, and when i run calculations, there is error ?

how to fix this error, from phq_readin : error # 1 DFPT with the Blochl correction is not implemented I am attaching my ph.in file.

23 July 2024 4,649 2 View