I have one probe sequence labelled with TAMRA as quencher. I want to replace the quencher from TAMRA to MGB. How can i do with current sequence?

07 September 2017 1 2K Report

I have already have selected the probe sequence specific to my gene but that is labelled with TAMRA as quencher but i want to label the same sequence with MGB quencher? will it change the probe sequence?

Konstantin A. Blagodatskikh

MGB will increase Tm of your probe, so you can make it much shorter.

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