Can anyone recommend a protocol for concentrae Adeno using PEG?

I need to a protocol for PEG-concentration of Adenovector or Adenovirus.

11 December 2015 9,107 1 View

Can you help me for Adenovirus CPE?

HI i transfected adenovector to hek293 cell line. i don't know Adenovirus CPE. this picture is hek 293 cell line 8 days post transfection. do you observe any CPE?

10 November 2015 5,150 4 View

Can you help me about problem with gateway technology and LR reaction?

09 October 2015 7,966 2 View

Can you help me with miRNA mimic transfection and negative control?

HiI want to transfect 10 nM, 20 nM and 30 nM of miR145 mimic to MCF-7 cell line. Then I want to transfect a plasmid containing miR 145 target sequences at the end of my gene. I want to evaluate...

09 October 2015 2,242 6 View

Can you help me about adenovector for cloning and virotherapy?

I am working on virotherapy using adenovirus.I want to return E1A adenovirus (including promoter + CDS) to pad/pl/dest vector (invitrogen).If I return E1A gene (from nucleotide 400 to 1700 of wild...

07 August 2015 7,118 1 View

What is your opinion about best moi of adenovirus for transfection of MCF-7 cell?

what is your opinion about best moi OF ADENOVIRUS for transfection of MCF-7 cell?

07 August 2015 9,135 2 View

Is it possible that supercoiled plasmid migrate slower than linearized in agarose gel ?

I have been trying to clone a 1200 bp insert to PENTR vector.After transformation and plasmid extraction, I run the super coiled and linearized vectors on the gel.The length of linearized vector...

06 July 2015 538 3 View

Can you help me for solving the problem of plasmid-insertion deletion in Ecoli during cloning?

I inserted 3131 bp to a plasmid with 3600 length by ECORI and BSP119I enzymesAfter transformation of bacteria (TOP10) and replication, I purified the plasmid.Plasmid digestion and sequencing...

05 June 2015 1,059 4 View

Can anyone advise me on solving the cloning problem?

I inserted 3131 bp to a plasmid with 3600 length by ECORI and BSP119I enzymes After transformation of bacteria (TOP10) and replication, I purified the plasmid. Plasmid digestion and sequencing...

04 May 2015 3,971 2 View

Can anyone advise me on solving the cloning problem?

I inserted 3131 bp to a plasmid with 3600 length by ECORI and BSP119I enzymesAfter transformation of bacteria (TOP10) and replication, I purified the plasmid.Plasmid digestion and sequencing...

04 May 2015 9,698 6 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Does anyone have any experience with the Motic EasyScan Pro Slide Scanner?

I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can...

11 February 2021 2,416 2 View

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Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

Where to buy optically clear, low viscosity, refractive index 1.5-1.4 Silicone Oil ?

For research purposes, it is required to have optically clear, low viscosity silicone oil with a refractive index of 1.5-1.4. It is going to be used in optoelectronic research. Online purchase is...

01 February 2021 9,160 3 View