i purchased Gateway®BP Clonase™ II Enzyme Mix (Invitrogen), but I do not need this enzyme.Does anyone of researchers needs this enzyme?
Yes I need it, please do you have Gateway®LR Clonase™ II Enzyme Mix? I need it too.
I need to a protocol for PEG-concentration of Adenovector or Adenovirus.
11 December 2015 9,180 1 View
HI i transfected adenovector to hek293 cell line. i don't know Adenovirus CPE. this picture is hek 293 cell line 8 days post transfection. do you observe any CPE?
10 November 2015 5,225 4 View
HiI want to transfect 10 nM, 20 nM and 30 nM of miR145 mimic to MCF-7 cell line. Then I want to transfect a plasmid containing miR 145 target sequences at the end of my gene. I want to evaluate...
09 October 2015 2,323 6 View
I have a problem with gateway technology and LR reaction.I Perform LR reaction with various vector concentrations but the reaction is not carryout. I did check LR enzyme and this enzyme worked...
09 October 2015 8,039 2 View
I am working on virotherapy using adenovirus.I want to return E1A adenovirus (including promoter + CDS) to pad/pl/dest vector (invitrogen).If I return E1A gene (from nucleotide 400 to 1700 of wild...
07 August 2015 7,272 1 View
what is your opinion about best moi OF ADENOVIRUS for transfection of MCF-7 cell?
07 August 2015 9,299 2 View
I have been trying to clone a 1200 bp insert to PENTR vector.After transformation and plasmid extraction, I run the super coiled and linearized vectors on the gel.The length of linearized vector...
06 July 2015 617 3 View
I inserted 3131 bp to a plasmid with 3600 length by ECORI and BSP119I enzymesAfter transformation of bacteria (TOP10) and replication, I purified the plasmid.Plasmid digestion and sequencing...
05 June 2015 1,132 4 View
I inserted 3131 bp to a plasmid with 3600 length by ECORI and BSP119I enzymes After transformation of bacteria (TOP10) and replication, I purified the plasmid. Plasmid digestion and sequencing...
04 May 2015 4,058 2 View
04 May 2015 9,774 6 View
I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...
01 August 2024 5,460 5 View
I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...
30 July 2024 2,483 5 View
The literature on Supply Chain Integration (SCI), Supply Chain Management (SCM), and collaborations, spanning several decades, deserves special attention. This topic, although addressed for a long...
23 July 2024 5,672 3 View
I am working on carbonic anhydrase immobilization into MOFs. I am facing problems with low enzyme loading.. The other issue is that when using p-NPA activity test to detect the activity of the...
20 July 2024 1,440 3 View
In many experiments where HK-2 cells were stimulated with TGF-β at 10 ng/mL for 48 hours, I observed that even in the control group, the baseline expression level of fibrosis proteins like FN1 and...
15 July 2024 1,929 2 View
Hello, Does anyone know where we could purchase a used NIRS system, for example, by Artinis or NIRx? Thank you. Greg
10 July 2024 8,325 0 View
Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...
10 July 2024 3,050 5 View
كيف يساهم انزيم الكاتلاز في عملية التمثيل الضوئي في النباتات؟
09 July 2024 7,512 1 View
ماهو دور انزيم الكاتليز في النباتات؟
09 July 2024 5,824 2 View
Penconazole 1. CGA 132465 2. CGA 190503 3. CGA 127841 Fluopyram 1. AE C656948-benzamide 2. AE C656948-pyridyl-acetic acid 3. AE C656948-carboxylic acid I have searched on sigma aldrich as well...
09 July 2024 3,414 2 View