22 Questions 8 Answers 0 Followers
Questions related from Mohammad Shayestehpour
I need to a protocol for PEG-concentration of Adenovector or Adenovirus.
12 December 2015 9,154 1 View
HI i transfected adenovector to hek293 cell line. i don't know Adenovirus CPE. this picture is hek 293 cell line 8 days post transfection. do you observe any CPE?
11 November 2015 5,199 4 View
HiI want to transfect 10 nM, 20 nM and 30 nM of miR145 mimic to MCF-7 cell line. Then I want to transfect a plasmid containing miR 145 target sequences at the end of my gene. I want to evaluate...
10 October 2015 2,292 6 View
I have a problem with gateway technology and LR reaction.I Perform LR reaction with various vector concentrations but the reaction is not carryout. I did check LR enzyme and this enzyme worked...
10 October 2015 8,013 2 View
I am working on virotherapy using adenovirus.I want to return E1A adenovirus (including promoter + CDS) to pad/pl/dest vector (invitrogen).If I return E1A gene (from nucleotide 400 to 1700 of wild...
08 August 2015 7,236 1 View
what is your opinion about best moi OF ADENOVIRUS for transfection of MCF-7 cell?
08 August 2015 9,250 2 View
I have been trying to clone a 1200 bp insert to PENTR vector.After transformation and plasmid extraction, I run the super coiled and linearized vectors on the gel.The length of linearized vector...
07 July 2015 591 3 View
I inserted 3131 bp to a plasmid with 3600 length by ECORI and BSP119I enzymesAfter transformation of bacteria (TOP10) and replication, I purified the plasmid.Plasmid digestion and sequencing...
06 June 2015 1,106 4 View
05 May 2015 9,742 6 View
I inserted 3131 bp to a plasmid with 3600 length by ECORI and BSP119I enzymes After transformation of bacteria (TOP10) and replication, I purified the plasmid. Plasmid digestion and sequencing...
05 May 2015 4,021 2 View
I inserted a 3131bp fragment (PCR product) to a vector backbone with 3600bp length by two enzymes ECORI and BSP. After plasmid extraction and linearization, the vector run on the Agarose gel. I...
04 April 2015 6,426 9 View
I inserted a 3131 bp fragment to a vector (3661bp) by ECORI and BSP119I enzymes. I performed the colony PCR and saved the positive colonies after cultivation. When I extracted cloned plasmid from...
04 April 2015 2,420 8 View
i cut a plasmid by HPAI enzyme (creation blunt end) and dephosphorylate simultaneously. after run on gel, 2 band observed. i nead to linearized plasmid for cloning. How can I decipher between...
04 April 2015 7,278 6 View
I have a vector with 5308bp length.I digested this vector by NotI and ECORI enzymes. I must be observe two bands 1346bp and 3962bp but I observed 1346bp and 2500bp. What I observed band at 2500bp...
04 April 2015 175 3 View
I want to transfect miR-145 MIMIC to cell. the target sequence of miR-145 is inserted to a vector and is transfected to this cell. indeed, i want to transfect micro RNA mimic and target to cell...
01 January 2015 6,423 3 View
12 December 2014 6,939 7 View
NK cells or interferon alpha?
12 December 2014 2,549 18 View
i purchased Gateway®BP Clonase™ II Enzyme Mix (Invitrogen), but I do not need this enzyme.Does anyone of researchers needs this enzyme?
12 December 2014 4,849 1 View
I must transfect miR-145 mature to MCF-7 cell line. What is the most effective method for transfection of Micro-RNA? What is the best protocol?
10 October 2014 3,185 7 View
I used from High Fidelity PCREnzyme Mix (thermo scientific:product number k0191) for PCR performing.My PCR product is 3131bp from HEK-293 cell line DNA.On the first day, I performed a PCR test but...
09 September 2014 5,139 14 View
I used from High Fidelity PCR Enzyme Mix (thermo scientific:product number k0191) for PCR performing.My PCR product is 3131bp from HEK-293 cell line DNA.On the first day, I performed PCR test but...
09 September 2014 9,783 3 View
I used from High Fidelity PCREnzyme Mix (thermo scientific:product number k0191) for PCR performing.My PCR product is 3131bp from HEK-293 cell line DNA.On the first day, I performed PCR test but...
09 September 2014 7,231 49 View
