12 December 2015 1 9K Report

I need to a protocol for PEG-concentration of Adenovector or Adenovirus.

More Mohammad Shayestehpour's questions See All
Can you help me for Adenovirus CPE?

HI i transfected adenovector to hek293 cell line. i don't know Adenovirus CPE. this picture is hek 293 cell line 8 days post transfection. do you observe any CPE?

10 November 2015 5,225 4 View

Can you help me with miRNA mimic transfection and negative control?

HiI want to transfect 10 nM, 20 nM and 30 nM of miR145 mimic to MCF-7 cell line. Then I want to transfect a plasmid containing miR 145 target sequences at the end of my gene. I want to evaluate...

09 October 2015 2,323 6 View

Can you help me about problem with gateway technology and LR reaction?

I have a problem with gateway technology and LR reaction.I Perform LR reaction with various vector concentrations but the reaction is not carryout. I  did check LR enzyme and this enzyme worked...

09 October 2015 8,039 2 View

Can you help me about adenovector for cloning and virotherapy?

I am working on virotherapy using adenovirus.I want to return E1A adenovirus (including promoter + CDS) to pad/pl/dest vector (invitrogen).If I return E1A gene (from nucleotide 400 to 1700 of wild...

07 August 2015 7,272 1 View

What is your opinion about best moi of adenovirus for transfection of MCF-7 cell?

what is your opinion about best moi OF ADENOVIRUS for transfection of MCF-7 cell?

07 August 2015 9,299 2 View

Is it possible that supercoiled plasmid migrate slower than linearized in agarose gel ?

I have been trying to clone a 1200 bp insert to PENTR vector.After transformation and plasmid extraction, I run the super coiled and linearized vectors on the gel.The length of linearized vector...

06 July 2015 617 3 View

Can you help me for solving the problem of plasmid-insertion deletion in Ecoli during cloning?

I inserted 3131 bp to a plasmid with 3600 length by ECORI and BSP119I enzymesAfter transformation of bacteria (TOP10) and replication, I purified the plasmid.Plasmid digestion and sequencing...

05 June 2015 1,132 4 View

Can anyone advise me on solving the cloning problem?

I inserted 3131 bp to a plasmid with 3600 length by ECORI and BSP119I enzymes After transformation of bacteria (TOP10) and replication, I purified the plasmid. Plasmid digestion and sequencing...

04 May 2015 4,058 2 View

Can anyone advise me on solving the cloning problem?

I inserted 3131 bp to a plasmid with 3600 length by ECORI and BSP119I enzymesAfter transformation of bacteria (TOP10) and replication, I purified the plasmid.Plasmid digestion and sequencing...

04 May 2015 9,774 6 View

Can anyone advise me on solving the cloning problem?

I inserted a 3131bp fragment (PCR product) to a vector backbone with 3600bp length by two enzymes ECORI and BSP. After plasmid extraction and linearization, the vector run on the Agarose gel. I...

03 April 2015 6,459 9 View

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