If your question is about how to make a solution from 100 mg/ml stock, then you can follow the C1V1=C2V2 equation to calculate how much enzyme solution (stock) you need to add for a final conc. of 1 mg/ml.
What if I prepare 1mg per ml from 100 mg per ml and keep it and use 1ml of this for addition in the medium?
Hi there, This is about dilution. The starting concentration is 100mg/mL (X) and the final is 1mg/mL (Y). The dilution factor is 100 (X/Y) then take one volume of the starting solution and put it into 99 volumes (X/Y - 1) of diluent! The general case is into brackets . If the final volume is imposed then divide it by the dilution factor to get the volume unit to consider.
Thanks for the response. I have one more doubt to clarify.
Every wet gram of minced tissue was digested in 5 ml of a digestion enzyme medium containing 1 mg/ml collagenase I in DMEM at 37 °C for 20 min each cycle in a shaking water bath.
So here if my DMEM medium for digestion is 5ml how much of 1 mg/ml collagenase I should I use?
For that you need an enzyme stock with a higher concentration. For example 100mg/mL and then you use 50µL of that for your 5mL DMEM
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